NRSE-sequence oligonucleotides (ODNs) block the downregulation of HCN1 channels by KA-induced seizure-like events in hippocampal organotypic slice cultures. ( A ) The hcn1 gene contains a highly conserved NRSF recognizing element (NRSE) within its first

2015-05-14

av M Larsson — NRSE: transposoner som cis-regulatoriska element kan påverka neurologisk funktion. Ett exempel Finishing the euchromatic sequence of the human genome. Sequences of 1000 The neutron resonance spin echo method (NRSE) option at FLEXX is particularly sequence heterochrony in xenarthran evolution,. För både NRSE- och H / O-förstärkningselementen klonades antingen uppströms eller 53 Among adenoviral sequences thought to interfere with heterologous a membrane targeting sequence, and whose promoter region contained both a neuron-restrictive silencing element (NRSE) and a cAMP-response element a membrane targeting sequence, and whose promoter region contained both a neuron-restrictive silencing element (NRSE) and a cAMP-response element massive whole-genome sequencing project in Alzheimer's disease.

We predicted that the Repressor Element Silencing Transcription Factor/Neuronal Restrictive Silencer Factor (REST/NRSF) regulates expression of ICP22 and ICP4. 2000-07-14 · The ∼21 base pair RE-1/NRSE sequence has been found in a number of neuron specific genes including the type II sodium channel , the SCG10 gene , synapsin I , NMDA receptor , and the cholinergic gene locus to name but a few. The NRSE sequence of pEGFP‐N2‐NRSE4× corresponds to the NRSE site of the human synapsin promoter and differs from the NRSE consensus sequence [] by one less conserved nucleotide (TTCAGCACCGCGGACAGTGCCTT) and to the NRSE dsRNA sequence [] by one further nucleotide outside the 17 nucleotides core sequence. In non-neuronal cells, neuron-restrictive silencer factor (NSRF) actively represses gene transcription via a sequence-specific DNA motif known as the neuron-restrictive silencer element (NRSE). This DNA motif has been identified in many genes that are specific markers for cells of neuronal and neuroendocrine lineage. Neuron-restrictive silencer factor has been shown to bind to the NRSE sequence within the MOR gene and play important roles in modulating the expression of the MOR gene.

2006-11-27 · From the sequence data analysis, we initially found the conserved Sp family binding site adjacent to NRSE in mouse, rat and human MOR gene that could regulate MOR gene expression. When treated with mithramycin A, a G/C box specific binding inhibitor, the mRNA level of MOR gene was increased in NRSF containing NS20Y cells but not in NRSF negative PC12 cells ( Figure 1 ).

So, a model is emerging whereby cells that are to become neurons activate the transcription of genes that contain the NRSE/RE1 sequence. These cells might then generate non-coding NRSE/RE1 dsRNAs

Produkt/tjänst Practical nRse. Lokalt företag.

Stéphanie De Gois, Leı̈la Houhou, Yoshio Oda, Marilys Corbex, Fabrice Pajak, Etienne Thévenot, Guilan Vodjdani, Jacques Mallet, Sylvie Berrard

2008-10-01 NRSE-containing sequence (positions -233 to -209) was placed upstream ofa minimal c-fos promoter fused to the luciferase structure gene (pNR-fLUC). Transient transfec-tion assays ofthis construct showed that a single copy of synapsin I NRSE repressed the fos promoter activity by 2-foldinHeLacells(Fig. 2). Additionofonemorecopyofthe 2000-09-01 In the NIH3T3 cell line, the RE-1/NRSE sequence leads to repression of reporter-gene activity, whereas introduction of exogenous REST4 leads to de-repression. These data indicate that REST4 does not act as a transcriptional repressor.

Use text editor or plasmid mapping software to view sequence. SnapGene File: Plasmid sequence and SnapGene enhanced annotations. Use with SnapGene software or the free Viewer to visualize additional data and align other sequences.
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2000-07-14 · The ∼21 base pair RE-1/NRSE sequence has been found in a number of neuron specific genes including the type II sodium channel , the SCG10 gene , synapsin I , NMDA receptor , and the cholinergic gene locus to name but a few. The NRSE sequence of pEGFP‐N2‐NRSE4× corresponds to the NRSE site of the human synapsin promoter and differs from the NRSE consensus sequence [] by one less conserved nucleotide (TTCAGCACCGCGGACAGTGCCTT) and to the NRSE dsRNA sequence [] by one further nucleotide outside the 17 nucleotides core sequence. In non-neuronal cells, neuron-restrictive silencer factor (NSRF) actively represses gene transcription via a sequence-specific DNA motif known as the neuron-restrictive silencer element (NRSE). This DNA motif has been identified in many genes that are specific markers for cells of neuronal and neuroendocrine lineage. Neuron-restrictive silencer factor has been shown to bind to the NRSE sequence within the MOR gene and play important roles in modulating the expression of the MOR gene.

Neuron-restrictive silencer factor has been shown to bind to the NRSE sequence within the MOR gene and play important roles in modulating the expression of the MOR gene.
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The neuron-restrictive silencer factor (NRSF) binds a DNA sequence element, called the neuron-restrictive silencer element (NRSE), that represses neuronal gene transcription in nonneuronal cells. Consensus NRSEs have been identified in 18 neuron-specific genes. Complementary DNA clones encoding a functional fragment of NRSF were isolated and found to encode a novel protein containing eight

The sequence analysis of HCN4 gene revealed the presence of a conserved NRSE motif, which is known to bind the transcriptional factor neuron-restrictive silencing factor (NRSF). A promoter analysis of HCN4 with rat cardiac myocytes identified the region inducing a basal transcriptional activity.

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In the NIH3T3 cell line, the RE-1/NRSE sequence leads to repression of reporter-gene activity, whereas introduction of exogenous REST4 leads to de-repression. These data indicate that REST4 does not act as a transcriptional repressor.

Moreover, several proteins, including RE1-silencing transcription factor or neuron-restrictive silencer factor, are recruited by this regulatory sequence.